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Graphing The Effect Of Variables On Enzyme Action Answers

The optimum temperature for the action of this enzyme is approximately 14. Understanding how enzymes function will make sense of why each factor needs to be fixed in place. Concluding Statement: Fill in the blanks. (b) This graph shows the effect of enzyme concentration on the reaction rate at a constant level of substrate. Enzyme Kinetics In this exercise we will look at the catalytic behavior of enzymes. Coordinate graphing sounds very dramatic but it is actually just a visual method for showing relationships between numbers. The effect size estimate the strength of the relationship between two variables in a population. What would be the effect on product formation if the enzyme were heated to a. reduce the rate of reaction for enzymes by binding to the protein and either blocking the spot where the substrate will bind or by making the enzyme’s shape incompatible with the substrate. 361072 0131248391 Draft Genome Sequences from a Novel Clade of Bacillus cereus sensu lato Strains Isolated from the International Spa. Once the equation is determined, the user can insert current values for the predictor (independent) variable to arrive at a. Study the pictographs on the worksheets and answer. The resulting curves showed the enzyme's optimum value for each factor and what happened to the reaction rate as the conditions moved away from the optimum. , Life: The Science of Biology , 4th Edition, by Sinauer Associates ( www. The Hypothesis. Each enzyme is specific for a particular reaction because its amino acid sequence is unique and causes it to have a unique three-dimensional structure. the independent variable) Which enzyme / substrate reaction to use; How to measure the enzyme activity (i. Industrial note on the effect of temperature on the rate of reaction. This lab deals with how an enzyme is affected by different concentrations of substrate--specifically what effect will different concentrations of a substrate (H 2 O 2. Factors Affecting Enzyme Activity by John Eed (Biology 1151) Abstract: e studied the effect of temperature, enzyme concentration and pH on enzyme activity. what is the optimal pH for lactase enzyme activity? 14. The graph below shows how two different enzymes react with a substrate as the temperature increases. At the end of this session, you must hand in answers to all the questions, along with print outs of any plots you created. to zero faster? Graph A 3. ) must be omitted. Controlled variables are usually not graphed because they should not change. All others are based on that, so Range will be 0 to 10. Much of this regulation is done by changing the activity of enzymes, which are biological catalysts. the formation of the mRNA by the help of RNA polymerase. The independent variable is the factor or condition that is changing or being changed by the experimenter. in graph form. in descriptive studies). a pH outside of its optimal range. Revision Questions. Watch the video about enzyme action by clicking on the pic of the monitor. Catecholase enzyme activity was measured through its absorption rate in a spectrophotometer, using light with a wavelength of 540 nm. Enzyme activity increases with increasing temperature, until it becomes hot enough to denature the protein (enzymes are proteins), then the activity would cease. In the paper airplane activity students select and build one of five different paper airplane designs and test them for distance and for time aloft. :) X-Axis should be IV. Collect data on enzyme reaction rate using an O2 gas sensor and data logger. The best thing to do in this section is to learn the basic shapes of each graph. 2 and answer questions about enzymes. An enzyme assay must be designed so that the observed activity is proportional to the amount of enzyme present in order that the enzyme concentration is the only limiting factor. Lactose Lab Answer Key Follow-up Questions: 1. The Simple Wave Simulator Interactive provides the learner with a virtual wave machine for exploring the nature of a wave, quantitative relationships between wavelength, frequency and speed, and comparisons between transverse waves such as those traveling through a rope and longitudinal waves such as sound. Competitive and noncompetitive inhibitors. Activity Sheets: Use the three worksheets and reference sheet to help students follow along with the activity. The effects of pH and temperature were studied for an enzyme-catalyzed reaction. She found three sources of information about enzymes used in biological washing powders. Higher or lower pH than the optimum slows the rate of activity. Chemical reactions are at the heart of all biological processes. 8 kcal/mole. The enzymes will not increase the rate of reactions as much as they would at 70 °C. Practical 1. Moreover, the data generated provide an opportunity to show students how to use computers to calculate fermentation rates and graph data in. Label and number the x and y-axis appropriately. Lab #_____ Graphing The Effect of Variables on Enzyme Action Graph the data tables below. The effect size estimate the strength of the relationship between two variables in a population. “What variables under the control of management have a causal effect on the amount of insurance sold by agents?” Once you have defined the research question more clearly, you are in a better position to develop a good hypothesis that provides an answer to the question. Which graph, A or B, shows the amount of substrate going to zero faster? 3. Seal test tube A1 with a stopper immediately after the enzyme is added. The punched out leaf disks will initially sink in a test tube of water but will float as photosynthesis occurs. Other enzymes, such as those in saliva, function best near neutrality. Information that is crucial for the understanding of the experiment (values of pH and. Investigate the variables that affect the drag force. The best way to remember the information in this chapter is to get a pen and paper and write down your answers before clicking on the Answer link which will take you to the correct page. You can see in the diagram above the basic action of an enzyme. A maltose molecule floats near and is captured at a specific site on the maltase enzyme. Specifically, you will be designing a detailed experiment to test the effect of Lactaid on milk. Scale the x-axis of your graph in units of 0. This lab allows students to investigate enzyme activity and the conditions that affect it. Consider the information provided in the “Why?” box and in Model 1 about these proteins. Dependent Variables are what happens BECAUSE of the Independent. 361072 0131248391 Draft Genome Sequences from a Novel Clade of Bacillus cereus sensu lato Strains Isolated from the International Spa. (1 mark) (ii) Plot the line which shows the effect of variable X on reaction rate with a fixed concentration of. Up to a point, the rate of the reaction will increase as a function of temperature because the substrates will collide more frequently with the enzyme active site. We tried to locate some good of Enzyme Graphing Worksheet Answer Key with Basics Of Enzyme Kinetics Graphs Article image to suit your needs. Enzyme is a protein composed of amino acids which are bind together by a. These powders contain protease enzymes. The cofactor of catechol oxidase is a copper ion, which can be removed by changing the pH surrounding the enzyme. Still other enzymes such as lipases function best at basic pH levels. Chemical reactions are at the heart of all biological processes. When designing an experiment to test the effect of factors affecting enzyme activity, the three key decisions to be made are: Which factor to investigate (i. The substrate solution was 1% catechol in water. You can use the following simulation to look at the effect that pH and substrate. 4: Investigating the effect of temperature on the action of an enzyme As temperature increases from 0°C to 40°C, the time taken for starch digestion decreases. If both graphs A and B show the rate of an enzyme, which enzyme is more effective? Justify your answer. Keywords: enzymes. O 2 gas was collected using the experimental setup described in the materials and methods section, the enzyme Catalase in chicken liver, and different temperatures. Type I fibres are more suited to prolonged activity as they are more efficient than type II fibres and have a greater reliance on oxidative metabolism of fatty acids and glycogen. Objective: To investigate the influence of substrate concentration over enzyme activity. We recorded the slopes of the resulting lines and graphed the average reaction rates (y -- here, the dependent variable) against the factor being tested (x, which was our independent variable). The active site is the portion of the enzyme that interacts with the substrate, so that any substance that blocks or changes the shape of the active site affect the activity of the enzyme. Gibberellic Acid and Plant Height Questions Gibberellic Acid (0. Still other enzymes such as lipases function best at basic pH levels. Answer the questions on the Lab Worksheet. Moreover, the data generated provide an opportunity to show students how to use computers to calculate fermentation rates and graph data in. Write a hypothesis for the effect of pH on catalase/enzymes. Which graph, A or B, shows the amount of substrate going to zero faster? 3. This also means activity decreases at colder temperatures. Enzymes have an optimum pH where they function the best. The data below summarizes the results of a scientific experiment on the effects of a growth hormone (gibberellic acid) on plant height. The purpose of this experiment is to analyze the effect of multiple variables to determine if they have an effect on the rate at which catalase catalyzes H 2 2. Use the graph below to answer questions 1-3. That is, the variables may be continuous, ordinal, interval, or. Graphing: Pictographs. At the end of this session, you must hand in answers to all the questions, along with print outs of any plots you created. Record the reaction rate using the Logger Pro software. What is the rate after 50 seconds? Why is it different from the initial rate? c. TYPE or use Blue or Black ink with COMPLETE SENTENCES. Biochemistry Activity #9 page 4 4. 02 sec and an amplitude of 240 V. Heat is required to break the bonds holding the molecules in the solid together. Take an apple and slice it into at least three pieces. What was the difference in activity between test tubes 1 and 2? Tube 2 had greater activity because there was no bile to inhibit the enzyme function. Construct a proper graph in Excel using the data on pg. It should serve as a tool to extend content. All of the above apply to an enzyme 1. Each enzyme is specific for a particular reaction because its amino acid sequence is unique and causes it to have a unique three-dimensional structure. Bottom - consequently, an enzyme's optimal rate of reaction is at an intermediate temperature. Lactase is a digestive enzyme that catalyzes the break down of lactose, a sugar found in milk. What is the dependent variable? 3. The enzyme solution was approximately 20 grams of potato in 5 L of water (0. two experiments — effect of pH and temperature on enzyme activity — should be included in this section. D While the peak of the graph is near 45°C, the graph does not specify that the enzyme activity is related to human. Read through the Student Activity Guide and consider the following questions. The effect of the pH on the activity of these enzymes can be determined by analyzing the data below. temperature of 100 oC for 10 minutes before repeating the. You should also examine the specificity of an enzyme to a specific substrate. A cofacter is the non-protein portion of an enzyme that is required for the enzyme to work. E represents the enzyme catalyzing the reaction; S, the substrate, the substance being modified; and P, the product of the reaction. Test what types of tissues contain the enzyme catalase. Ten taxis (enzyme molecules) are waiting at a taxi stand to take people (substrate) on a 10-minute trip to a concert hall, one passenger at a time. These products can be taken in pill form. Enzymatic Activity of Lactase OBJECTIVE The purpose of this lab is to explore the properties of the enzyme lactase. The graph also indicates the enzymatic activity decreased sharply when the pH. The reaction used in this laboratory exercise is the decomposition of hydrogen peroxide and the enzyme used is catalase. The aim of this activity is to investigate the effect of a reduction in enzyme concentration on the initial rate of reaction. In the "Charts" section of the Insert toolbar, click the visual representation of the type of graph that you want to use. the formation of the mRNA by the help of RNA polymerase. You can do a table of values and join the. Activity 1: Enzyme Activity. These reactions. what is the optimal pH for lactase enzyme activity? 14. Cite Popular Answers (1). Enzyme activity refers to the general catalytic properties of an enzyme, and enzyme assays are standardized procedures for measuring the amounts of specific enzymes in a sample. The body must regulate precisely all the chemical reactions going on in order to maintain life. Test the effect of concentration on enzyme activity. K eq depends only on the difference in energy level between reactants and products. Specific variables will be changed by calculated amounts to gauge their individual effects on the rate and amount of reaction that occurs. 4 - Effect of temperature on the action of an enzyme Procedure. Both lines on the graph finish at the same place. The reaction is as. The first piece of information was about Savinase, which is made by a company called. Information that is crucial for the understanding of the experiment (values of pH and. The enzymes most significantly impacted by alcohol consumption are found in your liver. Rates of Reaction. LABORATORY REPORT. speeds up a reaction by lower the activation energy. 1) LABEL each axis, 2) Create a SCALE for each axis, 3) PLOT your points. Part 2 - Effect of Temperature on Enzyme Function. The enzyme was catalase (100 units/ml). Many chemical substances can interact with the enzyme or substrate to inhibit enzyme activity. In this experiment, you will observe the enzyme peroxidase (catalase) speed up the breakdown of hydrogen peroxide into water and oxygen gas. Coordinate Graphing. Heat is required to break the bonds holding the molecules in the solid together. Read through the Student Activity Guide and consider the following questions. The topic with functions that we need to deal with is combining functions. The independent variable is graphed on the x-axis. Two important digestive enzymes for proteins are pepsin and trypsin. Plot of enzyme activity as a function of pH for several enzymes. graph data to determine relationships and trends; identify the following trends in the periodic table: Shielding effect is constant across the period and increases within given groups from top to bottom. an Area Graph. Temperature in oC. Label the graph with dependent and independent variables where they should be. Tests can be preformed to determine the effects of different conditions on the enzyme function. Identify the dependent and independent variables (hint: there are two independent variables you investigated, but only one dependent variable. Enzymes are a linear chain of amino acids, which give rise to a three-dimensional structure. Ask students to form small groups and to recall earlier work on enzyme action involving enzyme kinetics and the lock and key mechanism. Study your graphs and determine the optimal pH and temperature for assaying maltase activity. He also explains how temperature and pH could affect the rate of a reaction. The effects of pH and temperature were studied for an enzyme-catalyzed reaction. GeoGebra Math Apps Get our free online math tools for graphing, geometry, 3D, and more!. At too high a temperature enzymes denature and break down, therefore losing their functionality. In Cell A1 type Temperature, in Cell B1 type Enzyme A, and in Cell C1 type Enzyme B. It is a two step process. _____ _____ 4. sNァ・/title> 0 moves it up; C < 0 moves it down We can move it left or right by adding a constant to the x-value: g(x) = (x+C) 2. In this lab, you will investigate the action of the enzyme lactase. It is the result of what happens because of the independent variable. Factors Affecting Enzyme Activity by John Eed (Biology 1151) Abstract: e studied the effect of temperature, enzyme concentration and pH on enzyme activity. ,To,investigate,effect,of,a,substance,on,plant. Activity Sheets: Use the three worksheets and reference sheet to help students follow along with the activity. A pressure probe connected to an interface device and graphing calculator will measure the amount of oxygen gas produced. MS Excel can be used to calculate Vmax by selecting Trendline options. Explain and discuss the effect of environment on enzyme activity, on both the organismal and molecular levels. Substrate(s) bind in the active site 2. A pressure probe connected to an interface device and graphing calculator will measure the amount of oxygen gas produced. Enzyme inhibition: the effect of end product, phosphate, on the enzyme phosphatase. Up to a point, the rate of the reaction will increase as a function of temperature because the substrates will collide more frequently with the enzyme active site. When designing an experiment to test the effect of factors affecting enzyme activity, the three key decisions to be made are: Which factor to investigate (i. The effect of temperature on the relative rate of action of an enzyme is represented in the graph below. The rate of enzyme activity can be interfered with or prevented completely through the interaction of inhibitors. Create a graph for each factor and its time interval and display the Vmax below each graph. The following results were obtained. A POGIL activity guides students through an exploration to construct, deepen, refine, and/or integrate understanding of relevant disciplinary content. the enzyme activity is the dependent variable, the temperature, pH and substrate concentration are control variables and the enzyme concentration is the. In[7]:= We can see the functional form of the Gaussian distribution by giving NormalDistribution symbolic values. 1 molar solution was used in all experiments. A water bath has been prepared at a temperature in the range of 5 C with ice in a bucket labeled. What is the rate after 50 seconds? Why is it different from the initial rate? c. Use Excel or another graphing program to create a graph of the change in balloon circumference over time for each different temperature. Figure: Temperature and Enzyme Activity. Right before you start the activity use a spoon to remove the cubes. TYPE or use Blue or Black ink with COMPLETE SENTENCES. Enclosed is a Chart depicting enzyme activity at different temperatures, 1 Bar graph that contains the correct data, 4 Bar graphs that have one incorrect data point each, 1 Line graph that contains the correct data, and 4 Line graphs that have one incorrect data point each. Objective: To investigate the influence of substrate concentration over enzyme activity. Graph 1 shows the raw data for the effect of changing temperature on the rate of lipase activity 6 IB Biology HL Name: Yoojin Lee Candidate Number: 002213-067 pH(Âą0. to determine the effect of substrate and enzyme concentration rates of reaction Background information: Hydrogen peroxide (H 2 O 2 ) is a common but poisonous by-product of cellular metabolism, but H 2 O 2 does not accumulate in cells because it is decomposed into water and oxygen gas. These products can be taken in pill form. Graph showing the effect of changing pH on enzyme activity There are five main ways that enzyme activity is controlled in the cell. As the name implies, an inhibitor inhibits enzyme activity. Label and number the x and y-axis appropriately. The protein nature of the enzymes makes them extremely sensitive to thermal changes. The effect of temperature on the relative rate of action of an enzyme is represented in the graph. Find and study online flashcards and class notes at home or on your phone. The enzymes most significantly impacted by alcohol consumption are found in your liver. So, at a certain temperature, the enzymes start to denature, and any further increase in temperature will not help the enzymes activity any more. 4 - Effect of temperature on the action of an enzyme Procedure. In this Experiment, which variable is the dependent variable and which is the independent variable (1 pts)? The dependent variable is the Molecules of Product Formed per minute. Base your answers on the information and diagram below, which represent the changes in potential energy that occur during the given reaction. Write a hypothesis for the effect of pH on catalase/enzymes. It is satisfied only when the reaction is zero order. Enzyme Graphing Worksheet Answer Key Along with 26 New Enzyme Graphing Worksheet Answer Key. in descriptive studies). Scientists collect data on the dependent variable to see how it responds to the change made to the independent variable. Analyze a rate curve to find the rate of reaction. " A generic equation for complex formation is as follows: Enzymes do not: Change the equilibrium constant for a reaction. Alternately, you can. 4) CIRCLE each enzyme as directed. Investigate the effect of pH on the rate of reaction of amylase enzyme. Test what effect the chemical reaction has on the enzyme itself. Explore math with our beautiful, free online graphing calculator. temperature of 100 oC for 10 minutes before repeating the. These products can be taken in pill form. For both Graphs: Dependent Variable (thing you measured) (Y axis) is “Relative Rate of Enzyme Action” You will put down reaction rate from your data table. In an enzyme-catalyzed reaction, the substance to be … Continue reading "Enzyme. We added naphthalene for two. Use variable cost in a sentence “ A variable cost in manufacturing would be labor, which will increase when product demand increases, and will decrease when demand decreases. Assessment Rubric for Performance Assessment (doc). Part 1: Temperature and Enzyme Activity. Still other enzymes such as lipases function best at basic pH levels. speeds up a reaction by lower the activation energy. enzymes The effect of temperature on the relative rate of action of an enzyme is represented in the graph below. The active site is the portion of the enzyme that interacts with the substrate, so that any substance that blocks or changes the shape of the active site affect the activity of the enzyme. Use Excel or another graphing program to create a graph of the change in balloon circumference over time for each different temperature. What is the rate after 50 seconds? Why is it different from the initial rate? c. We isolate the enzyme from either plant or animal cells and compare how well the two enzymes work in the lab. In[7]:= We can see the functional form of the Gaussian distribution by giving NormalDistribution symbolic values. You can do a table of values and join the. Rates of Reaction. pH has a marked effect on the velocity of enzyme-catalyzed reactions. Enclosed is a Chart depicting enzyme activity at different temperatures, 1 Bar graph that contains the correct data, 4 Bar graphs that have one incorrect data point each, 1 Line graph that contains the correct data, and 4 Line graphs that have one incorrect data point each. Scale the x-axis of your graph in units of 0. Test the effect of concentration on enzyme activity. What is the effect of temperature on enzyme activity? a. What is the effect of substrate concentration on enzyme activity?. It is satisfied only when the reaction is zero order. C > 0 moves it up; C < 0 moves it down We can move it left or right by adding a constant to the x-value: g(x) = (x+C) 2. Used as a comparison to see if the experiment is working (independent variable has any effect) Experimental question Test Control (control group) Does fertilizer make a. Liver contains a specific enzyme called catalase. Here it is. Then it is only a matter of considering what effect the variables a, b and c are having. ” The description had to be clearly distinguishable from the rest of the answer and not simply implied in another part of the response. Remember, Part A was arbitrarily set to “4”. IV: Amount of yeast, pH level, either cold or heat. 1mL turnip extract concentration was picked as the most linear which had the highest R 2 value of 0. This is especially useful to visualise the effects that heat has on the activity of enzymes. The Simple Wave Simulator Interactive provides the learner with a virtual wave machine for exploring the nature of a wave, quantitative relationships between wavelength, frequency and speed, and comparisons between transverse waves such as those traveling through a rope and longitudinal waves such as sound. Used as a comparison to see if the experiment is working (independent variable has any effect) Experimental question Test Control (control group) Does fertilizer make a. Let’s consider an analogy. The graph in Figure 5. , Life: The Science of Biology , 4th Edition, by Sinauer Associates ( www. pH is the measure of H+ ions. Your function is only calculated between pH 9 and 12; therefore, you should just show these pH in your graph. In the graph you created in the lab simulation with your data: a. PAPER AIRPLANE ACTIVITY Overview. ) The Scales for each Variable: In constructing a graph, one needs to know where to plot the points representing the data. The enzyme solution was approximately 20 grams of potato in 5 L of water (0. Using this system, students can study effects of a wide range of parameters on the fermentation process, and experiments can be easily replicated within a single lab period. Activity 1: Enzyme Activity. Students will safely handle laboratory equipment and chemicals. Guidance/International mindedness: Guidance: Students should be able to sketch graphs to show the expected effects of temperature, pH and substrate concentration on the activity of enzymes. 8 for a numerical-quantitative data interpretation for a numerical-quantitative data interpretation AND the introduction page. The graph also indicates the enzymatic activity decreased sharply when the pH. The molecule s that enzymes work with are called. The enzyme solution was approximately 20 grams of potato in 5 L of water (0. However it would be wrong to. Read the bar graphs on the worksheets and answer questions. In this investigation, you and your group will study several factors that affect the activity of enzymes. The action of pepstatin on the enzyme rennin is an example of a) competitive inhibition b) reversible competitive inhibition. Ask students to form small groups and to recall earlier work on enzyme action involving enzyme kinetics and the lock and key mechanism. Test the effect of concentration on enzyme activity. In industry you would try to run the reaction at the highest economic temperature. Take one slice and put it in a bowl of shallow water -- just enough water to completely cover the slice of apple. Figure: Temperature and Enzyme Activity. Specifically, you will be designing a detailed experiment to test the effect of Lactaid on milk. If the amount of O 2 produced after one minute of reaction time is 32 mL, then the enzyme activity is: 32 mL/1 min= 32 mL/min. Enzyme A changes faster than B. Lesson is fully resourced and ready to use. Study the pictographs on the worksheets and answer. Why does enzyme activity increase on the left side of the graph? 3. The rest of the questions that are answered: Experiment 1: Enzymes In Food Data Tables and Post-Lab Assessment Table 1: Substance vs. In your answer, include an explanation of each of the variables used to calculate the unemployment rate. That alternative route has a lower activation energy. Scientists collect data on the dependent variable to see how it responds to the change made to the independent variable. Revision Questions. Label the graph with dependent and independent variables where they should be. (If this activity is not able to be accompanied by a math lesson on graphing, introduce the topic before the activity starts or perhaps after the class has recorded its data and worked through it as a group. When graphing, the independent variable is usually plotted along the x-axis, while the dependent variable is plotted along the y-axis. Enzyme Activity 6 Laying the Foundation in Biology 316 Enzyme Activity Measuring the Effect of Enzyme Concentration Enzymes are proteins that serve as biological catalysts in a wide variety of life sustaining chemical reactions that take place in cells. (13 points) Suppose that the United States imports most of our cellphones, and that a newly elected president decides to impose a tariff on imported cellphones. The dependent variable, which changes in response to the independent variable, is graphed on the y-axis. 8S Experimental investigation of a factor affecting enzyme activity. In the "Charts" section of the Insert toolbar, click the visual representation of the type of graph that you want to use. Enzyme is a protein composed of amino acids which are bind together by a. Give each a specific COLOR c) LABEL each enzyme on the graph for clarity. The animal enzyme has a Vmax 100 moles/sec of and a Km of 20 M. Change in enzyme concentration, temperature and pH showed significant change in peroxidase enzyme activity. The effect of sugar concentration on a-glucosidase reaction rate Alpha-glucosidase is an enzyme that breaks down sugar. The action of pepstatin on the enzyme rennin is an example of a) competitive inhibition b) reversible competitive inhibition. Construct a proper graph in Excel using the data on pg. Presented below are notes and comments regarding the laboratory material and preparation for the laboratory exercise. What is the effect of substrate concentration on enzyme activity?. There should be less digestion at a lower temperature due to the fact that the enzyme is most efficient at 37 deg. Base your answers on the information and diagram below, which represent the changes in potential energy that occur during the given reaction. Effect of Temperature on Enzyme A Action Effect of Temperature on Enzyme Action 30 o a. That is, the variables may be continuous, ordinal, interval, or. Enzymes are protein molecules that speed up chemical reactions. An enzyme assay must be designed so that the observed activity is proportional to the amount of enzyme present in order that the enzyme concentration is the only limiting factor. Thus the graph will show a temerature optimum - In the body, this is often around 37ºC. Explain and discuss the effect of environment on enzyme activity, on both the organismal and molecular levels. Students graph data collected about the rising of bread dough at different temperatures. pH has a marked effect on the velocity of enzyme-catalyzed reactions. Record the reaction rate using the Logger Pro software. This would best be seen as a line graph. Features of the Graph of Exponential Functions in the Form f(x) = b x or y = b x • The domain of f(x) = b x. The enzymes will require less energy to function than at 70 °C. Any student who draws a bar graph instead of a line graph will be denied credit for this part of the test. Watch the video about enzyme action by clicking on the pic of the monitor. Therefore, the enzyme bromelain denatures around _____ oC based on the results of. A pressure probe connected to an interface device and graphing calculator will measure the amount of oxygen gas produced. You will need to graph these data to observe your results. Hypothesis. The new value of the dependent variable is caused by and depends on the value of the independent variable. so looking at the structure of. The Hypothesis. For both Graphs: Dependent Variable (thing you measured) (Y axis) is “Relative Rate of Enzyme Action” You will put down reaction rate from your data table. Controlled Variables. Which of the following does NOT apply to an enzyme: 1. The effect of the pH on the activity of these enzymes can be determined by analyzing the data below. 26 The graph shows the effect of pH on the activity of two enzymes. Prepare a graph showing change in mass as a function of % salt. Plot the line which shows the effect of variable X on reaction rate with a fixed concentration of competitive inhibitor. Atomic radius decreases from left to right and increases from top to bottom within given groups.